Technical Data
Transferrin Receptor (TfR)
The human transferrin receptor (TfR) is a homodimeric type II membrane protein consisting of two identical 95kD subunits covalently linked by intermolecular disulfide bonds. The TfR has an extracellular domain of 671 amino acids, a single 28 residue transmembrane region and a 61 residue amino terminal cytoplasmic domain. Expression of the receptor is limited on normal tissue, however, highly expressed on the surface of some tumors. TfR is included in the class of ligand transport receptors which are internalized and clustered in coated pits. An important function of the receptor is to bind the serum transport protein transferrin (Tf) and mediate the uptake of iron into the cell. A growing area of research involves the use of monoclonal anti-TF receptor antibodies to inhibit the uptake of iron and thus block tumor cell growth.

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence/ Immunocytochemistry, Immunodepletion, Electron Microscopy, Immunogold-labeling and Iron uptake research. Other applications have not been tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 1ug/ml
Immunoprecipitation: 2-5ug/IP rxn
Immunohistochemistry (fixed, paraffin-embedded tissue): 1-5ug/ml
Immunofluorescence (IC): 1-5ug/ml
Optimal dilutions to be determined by the researcher.

Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.  
MabIgG16A202Affinity Purified
100ug-20°CBlue IceHumanMouse
Recombinant protein corresponding to human transferrin receptor. Species Sequence Homology: chicken, mouse, rat, Chinese hamster. Exhibits crossreactivity due to conserved amino acid sequences in the cytoplasmic tail.
Purified by affinity chromatography
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Recognizes aa3-28 of the human transferrin receptor (TfR) tail.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. White, S., et al., Cancer Research: 50 (1990). 2. Cameron, P.L., et al., J. Cell Biol. 115(1): 151-164 (1991). 3. White, S., et al., Biochimica et Biophysica Acta: 1136 (1992). 4. Brcückner, K., et al., Neuron 22(3): 511-524 (1997). 5. Cosulich, S.C., et al., EMBO. J. 16: 6182-6191 (1997). 6. Futter, C.E., et al., J. Cell Biol. 141(3): 611-623 (1998). 7. Jahraus, A., et al., J. Biol. Chem. 273: 30,389-30,390 (1998). 8. Prekeris, R., et al., J. Cell Biol. 143(4): 957-971 (1998). 9. Santama, N., et al., EMBO J. 17: 5855-5867 (1998). 10. Steegmaier, M., et al., J. Biol. Chem. 273: 34,171-34,179 (1998). 11. Tampanaru-Sarmesiu, A., et al., Am. J. Pathol. 152(2): 413-422 (1998). 12. Attieh, Z.K., et al., J. Biol. Chem. 274(2): 1115-1123 (1999). 13. Beatty, W.L., et al., J. Cell Biol. 145: 689-698 (1999). 14. Franco, M., et al., EMBO J. 18: 1480-1491 (1999). 15. Graft, G.A., et al., J. Biol. Chem. 274(17): 12,043-12,048 (1999). 16. Waheed, A., et al., Proc. Natl. Acad. Sci. 96: 1579-1584 (1999).