Technical Data
Transforming Growth Factor alpha, Recombinant, Human (TGFa)
Molecular Biology Storage: -20CShipping: Blue Ice
Transforming Growth Factor-alpha (TGF-alpha) is a polypeptide growth factor which stimulates the proliferation of a wide range or epidermal and epithelial cells. TGF-alpha is produced by monocytes, keratinocytes, and many tissues. TGF-alpha has been shown to play a role in the autocrine growth of certain transformed cells. Human TGF-alpha is a 5.5kD protein containing 50aa residues.

Recombinant corresponding to human Transforming Growth Factor alpha expressed in E. coli.

Molecular Weight:

Endotoxin: <0.1ng/ug

Biological Activity:
ED50 0.2ng/ml (>5x10e6units/mg). The biological activity was determined by measuring the TGF-alpha dose dependent proliferation of mouse BALB/c 3T3 fibroblasts. The optimal concentration should be determined for each specific application.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to 100ug/ml with sterile ddH2O. Reconstituted product is stable for 6 months at -20C. For long term storage add 0.1% HSA or BSA, aliquot, and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~98% (SDS-PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophillized powder. No preservatives or additives added.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.