Technical Data
Transforming Growth Factor beta 1, Recombinant, Human (TGFb1)
Molecular Biology Storage: -20CShipping: Blue Ice
Transforming growth factor-betas (TGF betas) are multifunctional peptides that regulate growth and differentiation in a variety of cells. The transforming growth factor beta (TGF-beta) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms (TGF-beta 1, -beta 2 and -beta 3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues.

Transforming growth factor beta mediates many cell-cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta-1, TGF beta-2 and TGF beta-3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. This antibody reacts with the precursor and mature form of recombinant human TGF beta- 2 and to a lesser extent TGF beta-3, but does not cross react with TGF beta-1.

Recombinant protein corresponding to aa177-391 from human TGF-b1 purified by standard chromatographic techniques. Shows a 51kD band on SDS-PAGE (including tag).

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: It is recommended to add a carrrier protein (0.1% HSA or BSA) for long-term storage.

Molecular Weight:
Purity: 80% as determined by Analysis by RP-HPLC or SDS-PAGE
Concentration: ~0.2mg/ml
Form: Supplied as a liquid in 50mM Tris-acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Expression of interleukin 1-beta, transforming growth factor beta-1, and vascular endothelial growth factor in soft tissue over the implant before uncovering. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006 May;101(5):565-71 2. Association of transforming growth factor-beta 1 functional polymorphisms with natural clearance of hepatitis C virus. J Infect Dis 2006 May 15;193(10):1371-4 3. Loss of SOCS3 in T helper cells resulted in reduced immune responses and hyperproduction of interleukin 10 and transforming growth factor-beta 1.J Exp Med 2006 Apr 17;203(4):1021-31 4. Prostaglandin E2 enhances transforming growth factor-beta 1 and TGF- beta receptors synthesis: an in vivo and in vitro study. Prostaglandins Leukot Essent Fatty Acids 2006 Mar;74(3):183-92 5. Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture patterns. Cell Biol Int 2006 Apr;30(4):295-300 6. Influence of HBcAg in liver cell plasma on expression of transforming growth factor-beta 1 in liver tissue of low-grade chronic hepatitis B patients. World J Gastroenterol 2006 Jan 7;12(1):127-9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.