Technical Data
T8250-16
Transforming Growth Factor beta1 (TGF beta 1, TGFb1, Camurati Engelmann Disease, CED, Diaphyseal Dysplasia 1 Progressive, DPD1, TGFb1, Differentiation inhibiting factor, Cartilage-inducing factor)
Description:
TGF-b1, -2 and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-b1, -2 and -3 are disulfide-linked homodimers.

Applications:
Suitable for use in Capture ELISA, Neutralization, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Capture ELISA: 2-8ug/ml. Can be paired with T8250-16B (0.1-0.4ug/ml) as the detection antibody in sandwich ELISA.
Neutralization: 0.3-1ug/ml will neutralize 50% of the bioactivity in the presence of 0.25ng/ml of recombinant human TGFb1 and 7.5ng/ml recombinant mouse IL-4. Measured by its ability to neutralize TGF-b1 inhibition of IL-4 dependent proliferation in the HT-2 mouse T cell line.
Western Blot: 1ug/ml. Reducing conditions are not recommended.
Flow Cytometry (Intracellular): 2.5ug/ml labels 1x10e6 cells. Validated using PC-3 human prostate cancer cell line fixed with paraformaldehyde and permeablized with saporin.
Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin embedded sections of human prostate cancer tissue.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo
I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Ar2Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to human transforming growth factor beta 1 (TGF-b1) and latent TGF-b1 expressed in CHO cells (P01137).
Purity:
Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide.). Neutralizes the biological activity of TGF-b1 and TGF-b1.2. Species Crossreactivity: mouse and rat. In sandwich ELISA, <2% crossreactivity with recombinant human TGF-b3 and recombinant amphibian TGF-b5 was observed and no crossreactivity with recombinant porcine or recombinant human TGFb2 was observed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lin, et al., Cell 68: 775-785 (1992). 2. Tsang, M., et al., Cytokine 7: 389 (1995).