Technical Data
T8270-10
6,6'-Trehalose Dimycolate (Cord-Factor, TDM)
1mg
Molecular Biology Storage: 4°CShipping: Blue Ice
6,6‘-Trehalose Dimycolate (Cord Factor, source: Mycobacterium bovis, Bacillus Calmette Guérin) 6,6'-dimycoloyl- -D-trehalose (Mycobacterium bovis, BCG) is used as an immunoadjuvant for the induction and non-specific stimulation of antibody production against unrelated antigens. This results in an increased host resistance against infections and cancer, in the potentiation of vaccine immunogeneticity and in an increase of DNA vaccination efficacy.

6‘6-Trehalose dimycolate (TDM) exhibits strong immunomodulatory effects, greatly enhancing the efficacy of DNA vaccination, shown to facilitate single-shot prime-boost DNA vaccines (3). TDM from M. bovis has a lower granulomatogenic activity as compared to TDM from M. tuberculosis (5). Preinjection of TDM followed by antigen injection triggers elevated antibody production (2). TDM stimulates macrophages, enhances non-specific immune responses and stimulates the antigen-presenting ability (7, 10). TDM Exhibits anti-tumor activity (2). TDM induces the production of both Th1-type and Th2-type cytokines (IFN-alpha, IFN-beta, IFN-gamma, IL-4, IL-6, IL-10, IL-12, TNF-alpha) as well as chemokines (MCP-1, MIP-1alpha, IL-8) (4, 6, 8, 9,11). TDM upregulates MHC class II expression on macrophages and causes expansion of Th1 cells (9). TDM is non-toxic on organisms and serves as protection against killing from macrophages. TDM becomes antigenic and highly toxic, when exposed to lipid surfaces.


Solubility (50mg/ml):
Chloroform: methanol: water (90:10:1)

Specific Rotation:
+32 to +35º, c=1.

Quality Control: MALDI-TOF

Endotoxin Content: 10e-4 EU/ug (LAL)

Applications:
Suitable for immunoadjuvant use. Other applications have not been tested.

Recommended Dilutions: 100ug to1mg TDM/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store at 4°C in the dark. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Source: Mycobacterium bovis, Bacillus Calmette Guèrin
Purity: ~90% Purified by Thin layer chromatography.
Form: Supplied as a lyophillized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Bekierkunst, A., I. S. Levij, et al. (1971), Science 174: 1240-1242. 2. Bekierkunst, A., E. Yarkoni, et al. (1971), Infect. Immun. 4: 256-263. 3. de Paula, L., S. C.L., et al. (2007),Genet Vacc Ther. 24(5): 2. 4. Emoto, M., Y. Emoto, et al. (1999), Eur. J. Immunol. 29: 650-659. 5. Fujita, Y., Y. Okamoto, et al. (2007), Microb. Pathog. 43(1): 10-21. 6. Guillemard,E.,M.Geniteau-Legendre, et al. (1995), AntiviralRes. 28: 175-189. 7. Kierszenbaum, F., A. Zemian, et al. (1984), Infect. Immun. 43: 531-535. 8. Oswald, I. P., C. M. Dozois, et al. (1997), Infect. Immun. 65: 1793-1799. 9. Ryll, R., K. Watanabe, et al. (2001), Microbes Infect. 3: 611-619. 10. Yarkoni, E., L. P. Ruco, et al. (1979), Eur. J. Cancer 15: 1401-1407. 11. Ryll, R., Kumazawa, Y. et al. (2001), Microbiol. Immunol. 45 (12): 801-811

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.