Technical Data
T8665-13F
Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)
Description:
Cardiac Troponin T, a 36kD protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion oncentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit Troponin C (TnC), the tropomyosin-binding subunit Troponin T (TnT), and the inhibitory subunit Troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place Because of which myosin can not bind the actin in relaxed muscle. When calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of Troponin I. The letter I is given due to its inhibitory character.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Western Blot and Affinity Purification. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b2Q1100Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Bovine cardiac Troponin I.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes bovine Troponin I. Species Crossreactivity: human, rabbit, mouse, chicken, fish, frog heart and skeletal muscle.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Kehat, I., et al., Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiocytes. The Journal of Clinical Investigation 108: 407-414 (2001).