Technical Data
T8665-22C
Troponin T, Cardiac (cTnT)
Description:
Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I and to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.

Applications:
Suitable for use in ELISA, Immunoaffinity Purification and Western Blot. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Matched Pairs:
Capture: T8665-22C, T8665-22C
Detection: T8665-22B, T8665-22A
Suitable for use as the capture or solid phase antibody.
Matched to T8665-22A, T8665-22B or T8665-30A as the detection or conjugate (label) antibody.

Hybridoma:
Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11.BB.157Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~3mg/ml
Immunogen:
Free human cardiac Troponin T
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human cardiac Troponin T. Recognizes an epitope between aa60-70. Does not crossreact with human skeletal Troponin T. Species Crossreactivity: Bovine and chicken. Does not react with canine, feline, fish, porcine, mouse, goat, rabbit or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.