Technical Data
T8665-23A
Troponin T, Cardiac (cTnT)
Description:
Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.

Application:
Suitable for use in Electron Microscopy, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Cardiac muscle

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.590Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceRabbitMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified rabbit cardiac troponin T isoform (TnT4R). Epitope: FMPNLVPPKI (5 region of the cardiac gene exon 10). Cellular Localization: Mostly over the I bands in myofibrils.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T8665-23AX.
Specificity:
Recognizes the cardiac isoform of rabbit Troponin T (cTnT). Species Crossreactivity: canine, chicken, goldfish, guinea pig, human, mouse, porcine, rat and sparrow. Does not react with frog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Malouf, N.N., et al., J. Biol. Chem. 267(13): 9269-9274 (1992). 2. Saba, Z., et al., Circulation 94(3): 472-476 (1996). 3. Anderson, P.A., Current Opinion in Cardiology 10(1): 33-43 (1995). 4. Anderson, P.A., et al., Circulation Res. 76(4): 681-686 (1995). 5. Greig, A., Circulation Res. 74(1): 41-47 (1994). 6. McMahon, D.K., et al., Am. J. Physiol. 266(6 Pt. 1): C1795-C1802 (1994). 7. Anderson, P.A., et al., Basic Research in Cardiology, 87(1): 117-127 (1992). 8. Malouf, N.N., et al., J. Biol. Chem. 267(13): 9269-9274 (1992). 9. Anderson, P.A., et al., Circulation Res. 69(5): 1226-1233 (1991). 10. Nassar, R., Circulation Res. 69: 1470-1475 (1991).