Technical Data
Tryptic Soy Broth w/o Dextrose (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Dextrose Absent
Potassium Phosphate Dibasic 2.5
Sodium Chloride 5.0
Enzymatic Digest of Soybean Meal 3.0
Pancreatic Digest of Casein 17.0
Total: 27.5g/liter
Nutritive basal medium for fermentation studies when appropriate carbohydrates are added.

Appearance: Light beige, homogenous, free flowing powder
Solubility: Lght amber, clear, complete
pH: 7.3 0.4

Directions per Liter: Dissolve 27.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.