Technical Data
Media
Tryptose Blood Agar Base (Powder)
T9055
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/L
Tryptose10
Beef Extract3
Sodium Chloride 5
Agar15
Total:33
A general purpose blood agar base medium.

Appearance:
Light beige powder, homogeneous, free flowing.


Solubility:
Yellow to light beige, turbid, incomplete.
Note: Agar will dissolve upon heating.

pH:
7.2±0.2

Cultural Response:
The medium was prepared according to the directions with 5% sheep blood and inoculated with the organisms listed below (10-300cfu). Cultures were incubated aerobically at 35±2°C and examined for growth at 18-24 hours.

Escherichia coli ATCC 25922
Staphylococcus aureus ATC 25923
Streptococcus pneumoniae ATC 6305
Streptococcus pyogenes ATC 19615

Growth Hemolysis
Excellent ---
Excellent Beta Hemolysis
Excellent Alpha Hemolysis
Excellent Beta Hemolysis

Country of Origin:
The raw animal product used in the manufacturing of Tryptose Blood Agar Base is derived from casein originating in the USA, New Zealand and/or Australia where there have been no reported cases of bovine spongiform encephalopathy
(BSE). During processing of the raw material, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes at 105°C. The product is then dried at a minimum of 140°C.


Directions per Liter: Dissolve 33 grams in 800-900ml of ddH2O stirring gently with heating with frequent agitation and boil for 1 min. to completely dissolve. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Prepare 5-10% blood agar by aseptically adding the appropriate volume of sterile, defibrinated blood to melted, sterile agar medium, cooled to 45-50°C.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Graham, J.P. et al., (2009) Environmental Research 109:682-689.