Technical Data
T9127-03
TSLP (Thymic Stromal Lymphopoietin)
Description:
Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 2.5ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
A20 Cell Lysate (WB), Human Brain Tissue (IHC)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 19 amino acid near the center of human TSLP (Genbank accession NP_149024).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human TSLP. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ziegler SF and Liu Y-J. Nature Immunol. 2006; 7:709-14. 2. Sims JE, Williams DE, Morrissey PJ, et al. J. Exp. Med. 2000; 192:671-80. 3. Levin SD, Koelling RM, Friend SL, et al. J. Immunol. 1999; 162:677-83. 4. Quentmeier H, Drexler HG, Fleckenstein D, et al. Leukemia 2001; 15:1286-92.