		Technical Data
T9154-02	Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Description:
Microtubules are major cytoskeletal elements found in all eukaryotic cells. They are composed of a 110kD protein, tubulin, which is a dimer of two 55kD subunits. Two classes of subunits, a and b, are identified. Tubulin dimer is composed of a heterologous pair of these subunits. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	2Q467	Highly Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rat

Concentration:

~0.1mg/ml

Immunogen:

Purity:

Purified by Ion Exchange chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 10mM sodium azide.

Specificity:

Recognizes Tubulin. Reacts with the aa414-422 on alpha-tubulin. Species Crossreactivity: Human, microtubules in most eukaryotic cells including mammalian, yeast, slime molds and allium. Note: The antibody has a greater affinity for formaldehyde or glutaraldehyde fixed microtubules than native.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Breitling, F., et al., (1991) Gene 104: 147–153. 2. Breitling, F. & Little, M. (1986) J. Mol. Biol. 189: 367–370. 3. Brinley, B.R., et al., (1980) Int. Rev. Cytol. 63: 59–95. 4. Chang, M.T., et al., (1981) J. Cell Biol. 91: (2,t.2), 333a (abstract). 5. Clayton, l. & Lloyd, C.W. (1984) Eur. J. Cell. Biol. 34: 248–253. 6. Kilmartin, J.V., et al. (1982) J. Cell. Biol. 93: 576–582. 7. White, E., et al.,(1983) J. Cell. Biol. 97: 1,011–1,019. 8. Cumming, R. & Burgoyne, R.D. (1984) In: Techniques in Immunocytochemistry, Eds. Bullock, G.R. & Petrusz, P. Academic Press Inc. Vol.3 pp55–78.