		Technical Data
T9154-05A	Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Description:
Applications:
Suitable for use in Immunohistochemistry, ELISA, Western Blot and RIA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen.
ELISA: 10ug/ul as detection antibody
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-49H: IgG, F(ab’)2 (HRP) Pab Rb xRt

Fusion Partners:
Spleen cells from immunized LOU rats were fused with cells of the rat YB2/0 myeloma cell line.

Source:
Tissue Culture Supernatant

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	3H3085	Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		4°C (-20°C Glycerol)	Blue Ice		Rat

Concentration:

~0.5mg/ml

Immunogen:

Yeast tubulin.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% sodium azide. before the addition of glycerol to 40%.

Specificity:

Specifically recognizes the alpha subunit of tubulin. The reactivity pattern is similar to that seen with similar clones. Species Crossreactivity: Yeast, avian and mammalian tubulin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kilmartin, J.V., et al., Rat monoclonal anti tubulin antibodies derived by using a new non-secreting rat cell line. J. Cell. Biol. 93: 576-582 (1982).