Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| As reported|
|Synthetic peptide corresponding to aa417-435 (DLVSEYQQYQDATADEQGE) of human b-Tubulin.|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.|
|Recognizes human beta Tubulin. Species Crossreactivity: bovine, chicken, canine, monkey, mouse, rat, xenopus.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Yeh,T.S., Hsieh,R.H., Shen,S.C., Wang,S.H., Tseng,M.J., Shih,C.M. and Lin,J.J. Nuclear betaII-tubulin associates with the activated notch receptor to modulate notch signaling. Cancer Res. 64 (22), 8334-8340 (2004). 2. Takahashi,M., Shiraishi,H., Ishibashi,Y., Blade,K.L., McDermott,P.J., Menick,D.R., Kuppuswamy,D. and Cooper,G. IV. Phenotypic consequences of beta1-tubulin expression and MAP4 decoration of microtubules in adult cardiocytes. Am. J. Physiol. Heart Circ. Physiol. 285 (5), H2072-H2083 (2003).|