Description:
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
Applications: Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution: Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Type | Isotype | Clone | Grade |
| Pab | IgG | | Affinity Purified |
|
| Concentration: |
| As reported |
| Immunogen: |
| Synthetic peptide corresponding to aa417-435 (DLVSEYQQYQDATADEQGE) of human b-Tubulin. |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. |
| Specificity: |
| Recognizes human beta Tubulin. Species Crossreactivity: bovine, chicken, canine, monkey, mouse, rat, xenopus. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Yeh,T.S., Hsieh,R.H., Shen,S.C., Wang,S.H., Tseng,M.J., Shih,C.M. and Lin,J.J. Nuclear betaII-tubulin associates with the activated notch receptor to modulate notch signaling. Cancer Res. 64 (22), 8334-8340 (2004). 2. Takahashi,M., Shiraishi,H., Ishibashi,Y., Blade,K.L., McDermott,P.J., Menick,D.R., Kuppuswamy,D. and Cooper,G. IV. Phenotypic consequences of beta1-tubulin expression and MAP4 decoration of microtubules in adult cardiocytes. Am. J. Physiol. Heart Circ. Physiol. 285 (5), H2072-H2083 (2003). |
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