Technical Data
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin
filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with a/b-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. g-tubulin is necessary to nucleate polymerization of tubulin subunits to form
microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome
alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Immunofluorescence (IC): 1:50
Flow Cytometry: 1:25
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to the sequence of human a-tubulin.
Purified by Protein A affinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Recognizes endogenous levels of huamn total a-tubulin protein at 52kD., Does not cross-react with recombinant b-tubulin. Species Crossreactivity: mouse, rat, monkey, bovine and Drosophilia.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell
Biol. 4, 938 947.