Technical Data
T9155-01Q
Tubulin beta, NT (Beta-tubulin, Tubulin beta Chain)
Description:
Tubulin beta is a 50kD globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape.

The evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule.

T9155-01Q is reported to partially inhibit microtubule assembly in vitro (2).

Applications:
Suitable for use in ELISA, Immunohistochemistry (frozen), and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM10F575Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IcePorcineMouse
Concentration:
~1mg/ml
Immunogen:
Tubulin beta from porcine brain.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes an epitope within the N-terminal region of porcine tubulin beta. Species Crossreactivity: human, plants, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krauhs, E. et al. (1981) Complete amino acid sequence of beta-tubulin from porcine brain. P.N.A.S. 78: 4156-4160. 2. Draber, P. et al. (1990) Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies. FEBS Lett. 262: 209-211.