Technical Data
Tubulin, beta (HRP) (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin
filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chro-mosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to the amino terminus of human beta-tubulin (KLH).
Supplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol, 50% glycerol. Labeled with HRP.
Recongnizes endogenous levels of human total Beta-tubulin protein and does not cross-react with recombinant alpha-tubulin. Species crossreactivity: mouse, rat, monkey, bovine, zebrafish. Sequence Homology: chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Westermann, S. and Weber, K. (2003) Nat. Rev. Mol.
Cell Biol. 4, 938-947.