Technical Data
T9155-50
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Description:
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Applications:
Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:400 for 20 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
LS174T, MAD109 cells. Skin or lung.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k0.N.593Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceChickenMouse
Concentration:
~0.2mg/ml
Immunogen:
Native chick brain microtubules. Epitope: aa426-450. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T9155-50X.
Specificity:
Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps (1). Does not block microtubule assembly (1). Does not inhibit polymerization or depolymerization of platelet tubulin in vitro (3). Blocks (70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species Crossreactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blose SH, et. al. Journal of Cell Biology, 1984, 98(3):847-58. 2. Breitling F, et. al. Journal of Molecular Biology, 1986, 189(2):367-70. 3. Berry S, et. al. Biochim Biophys Acta, 1989, 1012(1):46-56. 4. Roychowdhury S, et. al. Biochemistry, 1993, 32(18):4955-61.