Technical Data
Tubulin Polymerization Promoting Protein (TPPP, TPPP/p25, TPPP 1, TPPP1, 25kD Brain-specific Protein, Brain-specific Protein p25 alpha, Glycogen Synthase Kinase 3 (GSK3) Inhibitor p24, p24, p25, p25alpha)
Tubulin polymerization-promoting protein (TPPP) is an unfolded brain-specific protein that interacts with the tubulin/microtubule system, and is enriched in human pathological brain inclusions (1).TPPP can induce aggregation of alpha-synuclein, and can accumulate in
oligodendroglial cell bodies containing fibrillized alpha- synuclein multiple system atrophy (MSA), a neurodegenerative disease.TPPP is a constituent of myelin and a high-affinity ligand for myelin basic protein (MBP), and has been shown to colocalize with MBP in myelin in normal
human brains (2).

Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues in human TPPP
Supplied as liquid in 50mM Tris-Glycine (pH 7.4), 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Recognizes human TPPP. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hlavanda E, et al. J Biol Chem. 282(40):29531-9, 2007 2. Song YJ, et al. Am J Pathol. 171(4):1291-303, 2007