Technical Data
T9157-32
TBCA (Tubulin folding cofactor A)
Description:
Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b10C310Affinity Purified
SizeStorageShippingSourceHost
100ul20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant TBCA (1-108aa) purified from E. coli
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Specificity:
Recognizes human TBCA
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.HYPERLINK "http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Search&Term=%22Nolasco%20S%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlus" Nolasco S, et al., (2005) HYPERLINK "javascript:AL_get(this,%20'jour',%20'FEBS%20Lett.');" FEBS Lett 579(17):3515-3524
2. HYPERLINK "http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Search&Term=%22Tian%20G%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlus" Tian G, et al., (1996) Cell 86(2):287-296