Technical Data
T9157-32
TBCA (Tubulin folding cofactor A)
Description:
Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b10C310Affinity Purified
SizeStorageShippingSourceHost
100ul20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant TBCA (1-108aa) purified from E. coli
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Specificity:
Recognizes human TBCA
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.HYPERLINK "http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Search&Term=%22Nolasco%20S%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlus" Nolasco S, et al., (2005) HYPERLINK "javascript:AL_get(this,%20'jour',%20'FEBS%20Lett.');" FEBS Lett 579(17):3515-3524
2. HYPERLINK "http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Search&Term=%22Tian%20G%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlus" Tian G, et al., (1996) Cell 86(2):287-296