Technical Data
Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)
Molecular Biology Storage: -20CShipping: Blue Ice
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Molecular Weight: 17.3kD (157 Amino acid sequence)

Endotoxin Level: <0.1ng/ug

Biological Properties: Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells-this batch demonstrated an ED50 of < 0.5ng/ml.

Optimal working dilutions to be determined by researcher.
Purity: >98% by SDS PAGE and HPLC.
Concentration: ~5mg/ml
Form: Lyophilized in PBS. Reconstitute with 40ul distilled water.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.