Technical Data
Tumor Necrosis Factor alpha, Recombinant, Bovine (TNFa)
Molecular Biology Storage: 4C/-70CShipping: Blue Ice
Recombinant protein expressed in Pichia pastoris

Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response.

Biological Activity:
ED50 in the range of 1-10 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D.

Endotoxin Level:

Suitable for use in In Functional assays. Other applications not tested.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume with distilled water and store at -70C. Reconstituted product is stable for 12 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: >95% by SDS PAGE analysis.
Form: Supplied as a lyophilized powder. Preservative free

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.