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Technical Data |
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T9160-02E |
Tumor Necrosis Factor alpha, Recombinant, Bovine (TNFa) |
20ug |
| Growth Factors, Cytokines | Storage: 4°C/-70°CShipping: Blue Ice |
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Source: Recombinant protein expressed in Pichia pastoris Description: Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response. Biological Activity: ED50 in the range of 1-10 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D. Endotoxin Level: 0.05EU/ug. Applications: Suitable for use in In Functional assays. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume with distilled water and store at -70°C. Reconstituted product is stable for 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Purity: >95% by SDS PAGE analysis. Form: Supplied as a lyophilized powder. Preservative free Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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