Technical Data
T9160-10
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
10ug
50ug
50ug
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Technical Data |
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T9160-10 |
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa) |
10ug 50ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Tumor Necrosis Factor-alpha (TNFa) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-alpha is a 17.4kD protein containing 157 amino acid residues. Biological Activity: Human TNF-alpha is fully biologically active when compared to standards. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is 0.05ng/ml, corresponding to a specific activity of 2x10e7 units/mg. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 17.4kD |
Source: Human, recombinant expressed in E. coli Purity: 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug Form: Supplied as a lyophilized powder. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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US Biological application references: 1. Escobar, A. et al., (2005) Clin Exp Immunol. 142:555–568. 2. López, M.N. et al., (2007) Investigative Ophthalmology and Visual Science 48:1219-1227. 3. Cusack, J.C. et al., (2006) Clin Cancer Res. 12:6758-6764.
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