Technical Data
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
Molecular Biology Storage: -20CShipping: RT
Tumor Necrosis Factor-alpha (TNFa) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-alpha is a 17.4kD protein containing 157 amino acid residues.

Biological Activity:
Human TNF-alpha is fully biologically active when compared to standards. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is 0.05ng/ml, corresponding to a specific activity of 2x10e7 units/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human, recombinant expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug
Form: Supplied as a lyophilized powder.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Escobar, A. et al., (2005) Clin Exp Immunol. 142:555568. 2. Lpez, M.N. et al., (2007) Investigative Ophthalmology and Visual Science 48:1219-1227. 3. Cusack, J.C. et al., (2006) Clin Cancer Res. 12:6758-6764.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.