Technical Data
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
Molecular Biology Storage: -20CShipping: Blue Ice
TNF, alpha is an E. coli-derived recombinant protein corresponding to the C-terminal 157aa of human Tumor Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tumor cells.

ED50: 0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.

Recombinant corresponding to human TNF, alpha, expressed in E. coli.

Molecular Weight:
~17kD (157aa sequence)

Endotoxin: 0.1ng/ug

Suitable for use in ELISA, Functional Assays and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.2-0.4ng/well
Western Blot: 1.5-3ng/lane
Functional Assays: 0.05-20ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human
Purity: Purified: ~98% (SDS PAGE, HPLC).
Concentration: As reported
Form: Supplied as a lyophilized powder from 3mM tris, pH8.0. No preservative added. Reconstitute with 500ul sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Cernuda-Morollon, E. et al. (2001) 15-Deoxy-Delta 12,14-prostaglandin J2 inhibition of NF-kappaB-DNA binding through covalent modification of the p50 subunit. J. Biol.Chem. 276 (38): 35530-35536.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.