Technical Data
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)
Molecular Biology Storage: -20CShipping: RT
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

Recombinant murine TNF-alpha is a single, glycosylated, polypeptide chain containing 157 amino acids

Amino Acid Sequence:

Biological Activity:
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is <0.1ng/ml, corresponding to a Specific Activity of 10x10e6U/mg.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% (1mg/ml) solution.
2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Recombinant, murine expressed in E.coli.
Purity: 97% by RP-HPLC and SDS-PAGE.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to 0.1mg/ml.

intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Secretion of tumor necrosis factor-alpha by mouse peritoneal macrophages in the presence of dental sealers, sealapex and endomethasone. Perassi FT, Filho IB, Carlos IZ, J Endod 2004 Jul;30(7):534-7 2. Tumor necrosis factor-alpha drives 70% of cigarette smoke-induced emphysema in the mouse. Churg A, Wang RD, Wang X, Am J Respir Crit Care Med 2004 Sep 1;170(5):492-8 3. Genetic ablation of tumor necrosis factor-alpha (TNF-alpha) and pharmacological inhibition of TNF-synthesis attenuates MPTP toxicity in mouse striatum. Ferger B, Leng A, Hengerer B, J Neurochem 2004 May;89(4):822-33 4. Interleukin-1 beta (IL-1beta) induces tumor necrosis factor alpha (TNF-alpha) expression on mouse myeloid multipotent cell line 32D cl3 and inhibits their proliferation. Ledesma E, Martinez I, Rodriguez-Sosa M, Ramos G, Cytokine 2004 Apr 21;26(2):66-72 5. Mouse IgA inhibits cell growth by stimulating tumor necrosis factor-alpha production and apoptosis of macrophage cell lines. Reljic R, Crawford C, Ivanyi J, Int Immunol 2004 Apr;16(4):607-14 6. Molecular cloning and characterization of mouse LITAF cDNA: role in the regulation of tumor necrosis factor-alpha (TNF-alpha) gene expression. Bolcato-Bellemin AL, Mattei MG, Amar S, J Endotoxin Res 2004;10(1):15-23.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.