Technical Data
Tumor Necrosis Factor beta, Recombinant, Human (TNFb)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant TNF comprises a 171 amino acid fragment (35-205) corresponding to the mature TNF chain protein and is expressed in E. coli with an amino-terminal hexahistidine tag.

TNF is a potent multifunctional cytokine produced mainly by activated T and B lymphocytes. The protein has been shown to have a specific potent cytotoxic activity on tumor cells and a variety of other target cells as well as being a mediator of inflammation and immune function.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human, Recombinant, E. coli
Purity: Purified. 95% by SDS-PAGE.
Concentration: As reported
Form: Supplied as a liquid.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Ruddle, N.H. (1992) Curr. Opin. Immunol. 4(3) : 327-332.
Porter, A.G. (1990) FEMS Microbiol. Immunol. 2(4) : 193- 199.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.