Technical Data
T9161-20E
Tumor Necrosis Factor Receptor Type 2 (TNF-R2, CD120b, p75/p80, Tumor necrosis factor receptor superfamily member 1B precursor, Tumor necrosis factor receptor type II) (FITC)
Description:
Tumor Necrosis Factor Receptor Type 2 is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. The Tumor Necrosis Factor Receptor Type 2 molecule is shown to be responsible for stimulation of activated T-lymphocytes by TNF-alpha.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50
Immunohistochemistry (Frozen): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a9G296Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Recombinant extra cellular part of the TNF-receptor II.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes the extracellular part and the soluble receptor of mouse Tumor Necrosis Factor Receptor Type 2 (TNF-R2). Reactivity of soluble TNF-R2 is not inhibited by high concentrations of mouse TNF-alpha.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Tacchini-Cottier, F et al; Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice. J Immunol 1998, 160: 6182 2. Mennini, T et al; Glial activation and TNFR-I upregulation precedes motor dysfunction in the spinal cord of mnd mice. Cytokine 2004, 25: 127 3. Bigini, P et al; Immunohistochemical localization of TNFalpha and its receptors in the rodent central nervous system. Methods Mol Med 2004, 98: 73