Technical Data
Tumor Necrosis Factor Receptor 2, Recombinant, Human (TNFR2, CD120b)
Molecular Biology Storage: -20CShipping: Blue Ice
CD120b, otherwise known as sTNF receptor type II (sTNFR1I), is a member of the tumor necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE. CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumor necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFR1I acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor.

E. coli

Suitable for use in Functional Assays. Other applications not tested.

Biological Activity:
Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25ng/ml of recombinant human TNF-alpha is 0.125ug/ml.

Endotoxin: 1EU/ug

Molecular Weight: 18.9kD (174 amino acid residues)

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (SDS PAGE and HPLC).
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. Reconstitute with 200ul sterile ddH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.