Technical Data
Twisted Gastrulation Protein, Recombinant, Human (TSG)
Molecular Biology Storage: -20CShipping: Blue Ice
Twisted Gastrulation Protein (TSG) is a secreted BMP binding protein structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/Chordin complex. Recombinant human TSG is a 199 amino acid 22kD protein containing the BMP/TGF binding portion of the full length TSG protein.

Biological Activity:
Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is < 2.0 mg/ml of TSG.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, carrier protein, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:

Molecular Weight:
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses.
Form: Supplied as a lyophilized powder in 10mM sodium acetate, pH6.0. Reconcentrate with dH2O, carrier protein to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.