Technical Data
T9237-13
Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)
Description:
Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Bot: 1:1000 on 10ug of PC12 lysates.
Immunohistochemistry: 1:100-1:1000 at 4C, 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Not suitable for use on paraffin embedded, human tissue.
Immunofluorescence: 1:100-1:1000; Fixed with 4% PFA overnight at 4C.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Recommended Positive Control: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands

Negative Control: Liver

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes rat Tyrosine Hydroxylase at ~62kD. Species Crossreactivity: mouse, ferret, feline, human, squid, Drosophila, aplysia (marine mollusc). Expected this antibody will react with most mammalian and many non-mammalian species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tabuchi, K., et al., Science 318: 71-76 (2007). 2. Nunez, C., et al., Endocrinology 148: 5780-5793 (2007). 3. Pavon, N., et al., Biol. Psychiatry 59: 64-74 (2006). 4. Del Giacco, L., et al., BMC Dev. Biol. 6: 50 (2006). 5. Hollis, J., et al., J. Endocrinology 184: 393-406 (2005).