Technical Data
T9237-14
Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)
Description:
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000 detected Tyrosine Hydroxylase on 10ug of PC12 lysates. Recognizes a single band between 56-60kD.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, (HRP) Pab Gt xMo
I1904-19C: IgG, (HRP) Pab Gt xMo

Positive Control:
Brain (sympathetic nerve terminals), adrenal gland tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a2Q118Affinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes rat Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase). Species Crossreactivity: chicken, quail, mouse, human and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rohrer, H. et al. (1986) J. Neuroscience 6, 26162624. 2. Thibault, J. et al. (19B1) Biochem. Biophys. Res. Commun. 99, 960968.