Technical Data
U1000-03A
Ubiquitin
Description:
Ubiquitin is a 76 amino acid, highly conserved protein of about 8.5kD which is highly conserved evolutionarily. All plant and animal species are observed to express ubiquitin. Ubiquitin is produced in response to stress. The formation of ubiquitin-protein conjugates is a signal for the selective degradation of proteins. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Ubiquitin has been immunohistochemically localized to a number of pathological inclusions, including: Lewy bodies of Parkinson’s disease, neurofibrillary tangles of Alzheimer’s disease, Pick bodies of Pick’s disease, Mallory bodies of alcoholic liver disease, cytoplasmic bodies in specific myopathy, and Rosenthal fibers within astrocytes. All are heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Applications:
Suitable for use in Immunohistochemistry, Western Blot, ELISA, and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen and fixed): 1:1000
Immunofluorescence (frozen): 1:200-400
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Purified rabbit ubiquitin (glutaraldehyde).
Purity:
Serum
Form
Supplied as a liquid.
Specificity:
Recognizes ubiquitinated inclusion bodies and both the mono- and polyubiquitin forms. Species crossreactivity: human, bovine, mouse, chicken, Drosophila and C. elegans.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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