Technical Data
U1000-06P
Ubiquitin (Lys48)
Description:
Polyubiquitin chains linked from the Lys48 of one protein to the C-terminus of the other are the most common ubiquitin linkage. Lys48- linked ubiquitins are abundant in vivo and act as a universal signal for proteins targeted for proteosomal degradation.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Peptide Inhibition Assay and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:700 permeabilized Jurkat cells
Western Blot: 1:1000-1:5000
Immunoprecipitation: 10ul/100ug HeLa cell lysate
Immunohistochemistry: 1:100. Formalin fixed sections were heated in citrate buffer, pH 6.
Peptide Inhibition Assay: 1:1000 dilution with K48-multiubiquitin chains inhibited binding to HeLa cell lysate.
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-46E: IgG, H&L (HRP) Pab DkxRb
I1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab DkxRb

Positive Control:
Lys48 polyubiquitin chains

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9H284Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Human di-ubiquitin linked at Lys48.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin. Species Crossreactivity: mouse, rabbit, and rat. Based on sequence homology, broad species crossreactivity is expected.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Newton, K., et al. (2008) Cell 134: 668678. 2. Stewart, GS., et al. (2009) Cell 136: 420434. 3. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb2pea/pdb 4. Ikeda, F., & Dikic. I. EMBO Reports 9:536-542.