Technical Data
U1000-07D
Ubiquitin
Description:
Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transfered to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IkB, p53, cdc25a and bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response and apoptosis (4-7).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:1600. Antigen retrieval by heating in citric acid buffer is recommended.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the amino terminus of the human ubiquitin protein (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Specificity:
Recognizes human ubiquitin at ~9kD, polyubiquitin and ubiquitinated proteins. Species Crossreactivity: mouse, rat,. Species sequence homology: bovine, monkey, porcine, Drosophila, Xenopus, zebrafish-100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Ciechanover, A. (1998) EMBO J. 17, 71517160. 2) Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153E157. 3) Hochstrasser, M. (2000) Science 289, 563564. 4) Bernardi, R. et al. (2000) Oncogene 19, 24472454. 5) Aberle, H. et al. (1997) EMBO J. 16, 37973804. 6) Salomoni, P. and Pandolfi, P.P. (2002) Nat. Cell Biol. 4, E152E153. 7) Jesenberger, V. and Jentsch, S. (2002) Nat. Rev. Mol. Cell Biol. 3, 112121.