Technical Data
Vascular Endothelial Growth Factor, Recombinant, Mouse (VEGF)
Molecular Biology Storage: -20CShipping: RT
Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.

Recombinant Murine VEGF is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 39kD.

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Thr.

Biological Activity:
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-6.0ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile dH2O, 0.1% BSA or HSA. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight:
Source: E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form: Supplied as a lyophilized powder. No additives. Reconstitute to with sterile dH2O, 0.1% HSA or BSA to 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Graells J, Vinyals A, Llorens A, Fabra A, J Invest Dermatol 2004 Dec;123(6):1151-61 2. Hiratsuka S, Kataoka Y, Nakamura K, Maru Y, Mol Cell Biol 2005 Jan;25(1):355-63 3. Eubank TD, Roberts R, Wang Y, Immunity 2004 Dec;21(6):831-42 4. Loureiro RM, Maharaj AS, Muller WJ, Biochem Biophys Res Commun 2005 Jan 14;326(2):455-65 5. Emoto M, Charnock-Jones DS, Ishiguro M, Hachisuga T, Gynecol Oncol 2004 Dec;95(3):474-82 6. Belo AV, Barcelos LS, Ferreira MA, Cancer Invest 2004;22(5):723-9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.