Technical Data
VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Suitable for use in Neutralization, ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin-fixed paraffin embedded): 5-15ug/ml with a 30 minute incubation at RT or an overnight incubation at 4C.
Neutralization: 0.05-0.1ug/ml yields one-half maximal
inhibition [ND50] of the biological activity (10ng/ml).
ELISA (Indirect amd Sandwich): 0.5ug/ml (100ul/well). Used in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well of recombinant hVEGF.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. For long term storage, stored at -20C. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
PabIgGAffinity Purified
50ug4C (-20C Glycerol)Blue IceHumanGoat
Not determined
Highly purified (>98%) recombinant human VEGF.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile dH2O to a concentration 0.1-1mg/ml
Recognizes Vascular Endothelial Growth Factor (VEGF)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.