Technical Data
V2110-18V1
Vascular Endothelial Growth Factor Receptor 2 (VEGF Receptor 2, VEGFR 2, VEGFR2, VEGFR-2, VEGFR, CD309 Antigen, Fetal Liver Kinase 1, FLK1, Kinase Insert Domain Receptor, KDR, KRD1, Ly73, Protein Tyrosine Kinase Receptor FLK1)
Description:
VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerizes and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry, and Immunocytochemistry/Immunofluorescence. Other applications not tested.

Recommended Dilutions:
ELISA: 1-15ug/ml
Flow Cytometry: 3-15ug/ml
Immunocytochemistry/Immunofluorescence: 6-30ug/ml
Immunohistochemistry (Frozen, paraffin): 6-30ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19F485Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Recombinant human soluble extracellular VEGFR-2 (110kD) native protein. Cellular Localization: Type I membrane protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes native human VEGFR-2 in ELISA experiments and on the surface of different human cell types. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Frederick JR, Fitzpatrick JR 3rd, McCormick RC et al Stromal cell-derived factor-1alpha activation of tissue-engineered endothelial progenitor cell matrix enhances ventricular function after myocardial infarction by inducing neovasculogenesis Circulation 2010 Sep 14