Technical Data
V2130-16
Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin, VDBG)
Description:
Gc-globulin is a plasma protein produced in the liver. Amongst its ligands are vitamin D, thus Gc-globulin is also called vitamin D-binding protein. Gc-globulin is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood stream. The molecular mass of Gc-globulin is approximately 50kD. The concentration of Gc-globulin in human plasma is ~400ug/ml (1).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653: Female CF1 Balb/c mice immunized by intraperitoneal injection.

Culture Medium:
RPMI 1640 with 10% fetal calf serum.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k19E8Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Gc-globulin isolated from human plasma.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity:
Recognizes human Gc-globulin. Reacts strongly with Gc-globulin when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Masuda S, Okano T, Osawa K, Shinjo M, Suematsu T, Kobayashi T (1989) Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrosis. J Nutr Sci Vitaminol 35:225-34.