		Technical Data
V2130-16	Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin, VDBG)

Description:
Gc-globulin is a plasma protein produced in the liver. Amongst its ligands are vitamin D, thus Gc-globulin is also called vitamin D-binding protein. Gc-globulin is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood stream. The molecular mass of Gc-globulin is approximately 50kD. The concentration of Gc-globulin in human plasma is ~400ug/ml (1).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653: Female CF1 Balb/c mice immunized by intraperitoneal injection.

Culture Medium:
RPMI 1640 with 10% fetal calf serum.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2b,k	19E8	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Gc-globulin isolated from human plasma.

Purity:

Purified by Protein A/G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.

Specificity:

Recognizes human Gc-globulin. Reacts strongly with Gc-globulin when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Masuda S, Okano T, Osawa K, Shinjo M, Suematsu T, Kobayashi T (1989) Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrosis. J Nutr Sci Vitaminol 35:225-34.