Technical Data
V2150-54D
Vitellogenin (salmon)
Description:
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:100-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6D838Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Vitellogenin from plasma of 17-beta estradiol induced Atlantic salmon (Salmo salar).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes Vitellogenin from Atlantic salmon. Crossreacts with Vitellogenin from brown trout (Salmo trutta) but not with Vitellogenin from rainbow trout (Oncorhynchus mykiss), Arctic char (Salvelinus alpinus), Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus) or halibut (Hippoglossus hippoglossus).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.