Technical Data
Vitellogenin (Atlantic salmon)
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:100-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,kKB-1Affinity Purified
100ul-20CBlue IceMouse
Not determined
Vitellogenin from plasma of 17-beta estradiol induced Atlantic salmon (Salmo salar).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Recognizes Vitellogenin from Atlantic salmon (Salmo salar) and other Salmo spp. Crossreacts with Vitellogenin from brown trout (Salmo trutta) but not with Vitellogenin from rainbow trout (Oncorhynchus mykiss), Arctic char (Salvelinus alpinus), Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus) or halibut (Hippoglossus hippoglossus).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.