Technical Data
V2200-06A
Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)
Description:
Vitronectin is a 71kD secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably avB3) and thrombin. The 459 aa mature human Vitronectin shows 74% amino acid identity with mouse Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65kD and 10kD components.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 10ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b7H101Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Human Vitronectin purified from plasma (hVitronectin)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes human Vitronectin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.