Technical Data
W0481-01
WC8 (FITC)
Description:
WC8, a cell surface antigen, is a 160kD protein which is expressed by activated bovine T cells. WC8 is not present on resting lymphocytes, but can be induced on T cells following activation by alloantigens or lectins (1).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG19i133Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
~0.1mg/ml
Immunogen:
Con A stimulated bovine lymphocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 1% BSA. Labeled with FITC.
Specificity:
Recognizes the bovine WC8 cell surface antigen at ~160kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nthale, J.M. et al. (1993) Characterization of a late activation antigen defined by monoclonal antibodies of cluster BoWC8 (TC23). Vet. Immunol. Immunopathol. 39: 201-208. 2. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet. Immunol. Immunopathol. 39: 25-48.