Technical Data
W0481-74E
WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1) (FITC)
Description:
WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. CC101 immunoprecipitates a 215 kD molecule from bovine cells, and also recognizes the swine homolog of WC1, which is a 180kD molecule. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T-cell population that is also CD2-, CD4-, CD8-.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a10F580Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
~0.1mg/ml
Immunogen:
Con A stimulated bovine lymphocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes bovine WC1 cell surface antigen, expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. Species Crossreactivity: porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes. Immunology 81: 36-40. 2. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle Vet. Immunol. Immunopathol. 39: 25-48.