WC9 is expressed on bovine monocytes, platelets, granulocytes, peripheral B lymphocytes and a subset of T lymphocytes.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 1:10-1:50 10ul labels 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|Bovine WC9 cell surface antigen.|
|Purified by Protein G Chromatography.|
|Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (FITC).|
|Recognizes the bovine WC9 cell surface antigen. Species Crossreactivity: sheep.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Naessens, J. et al. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies. Vet. Immunol. Immunopathol. 39: 217-223. 2. Menge, C. et al. (2004) Bovine Ileal Intraepithelial Lymphocytes Represent Target Cells for Shiga Toxin 1 from Escherichia coli. Infect. Immun. 72: 1896–1905. 3. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet. Immunol. Immunopathol. 39: 283-290. 4. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet Immunol. Immunopathol. 39:25-48.|