WC14 is expressed on monocytes and granulocytes, and a subset of activated T cells.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
P3-NS1/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
|Theileria parva infected bovine lymphoblastoid cell line.|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|
|Recognizes the bovine WC14 cell surface antigen.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Naessens, J. et al. (1985) De novo expression of T cell markers on Theileria parva- transformed lymphoblasts in cattle. J. Immunol. 135: 4183-4188. 2. Muiya, P. et al. (1993) Expression of antigens on haemopoietic progenitor cells in bovine bone marrow. Vet Immunol Immunopathol. 39:237-48. 3. Naessens, J. et al. (1996) Biochemical analysis of preliminary clusters in the non-lineage panel. Vet. Immunol. Immunopathol. 52: 347-356. 4. Howard, C.J. et al. (1996) Ruminant cluster WC14. Vet. Immunol. Immunopathol. 52: 261-262.|