Technical Data
W1018-50E
West Nile Virus, Envelope (West Nile Virus E Protein, WNV Envelope Protein, Envelope Protein, Genome Polyprotein, Major Envelope Protein E, WNVgp1)
Description:
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (1). Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins (1). While the viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway (2), the highly glycosylated envelope protein plays a major role for WNV entry into target cells as this entry can be inhibited by using a recombinant domain III from the envelope glycoprotein (3). The WNV receptor has recently been identified as alpha v beta 3 integrin (4).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (Frozen): 1ug/ml
Immunohistochemistry (Paraffin-embedded): 1ug/ml Protease 3min RT is recommended
Optimal dilutions to be determined by the researcher.

Positive Control:
Transfected cells and WNV-Envelope protein in bird liver.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to 73-86, CPTMGEAHNEKRAD of West Nile virus envelope glycoprotein (NC_001563).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.05% sodium azide.
Specificity:
Recognizes West Nile Virus envelope glycoprotein E. Crossreacts with many species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smithburn JS, Hughes TP, Bruke AW, Paul JH. Am. J. Trop. Med. Hyg. 20: 471.