Technical Data
W1023-70
WFS1 (Wolfram Syndrome 1 (Wolframin), Wolfram Syndrome, WFRS, WFS, Wolframin, DFNA14, DFNA38, DFNA6, DIDMOAD)
Description:
WFS1 is a novel component of Wolfram syndrome, a rare form of juvenile diabetes. WFS1 plays an important role in maintaining homeostasis of the endoplasmic reticulum (ER) in the pancreas. It is normally up-regulated during insulin secretion, whereas inactivation of the protein can cause ER stress. Chronic ER stress is a major involvement in Wolfram syndrome.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000. Heat samples at 50°C for 30 minutes.
Immunoprecipitation: 2ul
Immunofluorescence: 1:200
Immunohistochemistry (Frozen): 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to 110 amino acid from the N-terminal region of the human WFS1. Cellular Localization: Endoplasmic reticulum
Purity:
Serum
Form
Supplied as a liquid.
Specificity:
Recognizes human WFS1 protein at ~100kD. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fonseca, SG, et al. J. Biol. Chem. 280: 39609-39615 (2005). 2. Takeda, K, et al. Hum Mol Gen. 10: 477-484 (2001).