Technical Data
WFS1 (Wolfram Syndrome 1 (Wolframin), Wolfram Syndrome, WFRS, WFS, Wolframin, DFNA14, DFNA38, DFNA6, DIDMOAD)
WFS1 is a novel component of Wolfram syndrome, a rare form of juvenile diabetes. WFS1 plays an important role in maintaining homeostasis of the endoplasmic reticulum (ER) in the pancreas. It is normally up-regulated during insulin secretion, whereas inactivation of the protein can cause ER stress. Chronic ER stress is a major involvement in Wolfram syndrome.

Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000 detects a band at ~100kD and ~200kD representing the monomer and dimer forms of WFS1.
Immunoprecipitation: 2ul
Immunofluorescence (IC): 1:200
Immunohistochemistry (Frozen): 1:1000
Immunohistochemistry (Paraffin): 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not Determined
A synthetic peptide corresponding to 110 amino acid from the N-terminal region of the human WFS1. Cellular Localization: Endoplasmic reticulum
Supplied as a liquid.
Recognizes human WFS1.Species crossreactivity: mouse, monkey and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fonseca, SG, et al. J. Biol. Chem. 280: 39609-39615 (2005). 2. Takeda, K, et al. Hum Mol Gen. 10: 477-484 (2001).