Technical Data
Wnt1 (Proto-oncogene Wnt-1, Proto-oncogene Int-1 Homolog, INT1)
Wnt1, a 40kD cyteine-rich secretory glycoprotein, is a highly conserved archetypal member of WNT protein family. Wnt1, originally identified as proto-oncogene, plays a significant role in oncogenesis and in various developmental processes including regulation of cell fate, cell survival and patterning during embryogenesis. Wnt1 binds to its FZD receptor on the cell surface resulting in activation of beta-Catenin and translocation into the nucleus. Activated beta-catenin makes a complex with TCF family of transcription factors in the nucleus resulting in transactivation of several genes including c-myc and Cyclin D1 who play pivotal role in cell survival and differentiation. Mutations in the members of Wnt1 signaling have been suggested in several cancer types.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa66-79 (RKQRRLIRQNPGIL) of human Wnt1.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human Wnt1. Species Crossreactivity: bovine, chicken, chimpanzee, canine, equine, mouse, rhesus monkey. Species sequence homology: pufferfish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. You Cancer Res 64; 3474-3478 (May 15, 2004) 2. He Oncogene 24; 3054-3058 (2005).