Technical Data
W3028-01
Wnt-5a (Protein Wnt-5a, Wnt5a, Wnt-5)
Description:
Wnt-5a is a 49kD glycoprotein member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its lack of transforming activity on C57MG cells. Genetic deletion of Wnt-5a interferes with developmental proximal-distal outgrowth of limbs and other structures. Wnt-5a can activate or inhibit b-catenin signaling depending on receptor context. Mature mouse Wnt-5a is a 342aa protein that contains 24 cysteines and four potential N-linked glycosylation sites. Mature mouse, rat and human Wnt-5a share 99% aa identity.

Applications:
Suitable for use in Immunohistochemistry, ELISA, and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 8-25ug/ml
Western Blot: 10ug/ml. Use non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2A11C65Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Purified, CHO-derived, recombinant mouse Wnt-5a corresponding to aa38-380, UniProt Accession #P22725.
Purity:
Purified from culture supernatant by Protein A for G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes mouse Wnt-5. Species Crossreactivity: human. By direct ELISA, no cross-reactivity observed with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11 and 16.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.