Technical Data
X-Gluc Cyclohexylammonium Salt (XGLUC)
Molecular Biology Storage: -20CShipping: RT
Indigo-blue chromogenic substrate used in the detection of beta-glucuronidase (GUS fusion marker) in E. coli for transcription and translation studies. Additional applications include bacterial contamination testing in water, meat, dairy, shellfish.

The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the B-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized color, making it useful in identifying GUS gene presence in most cell types1 and for the detection of the GUS gene fusion marker in plants. X-Gluc has reported applications in the detection of contaminated food samples such as meat, dairy, and shellfish products. X-Gluc also has clinical applications in the assessment of urinary tract infection by d etecting the pr esence of E. coli. It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples b y reducing false positives and negatives found with traditional methods.

Solubility (1% DMF/water 1/1): Colorless, clear, complete

Water (KF): 5.0%

Specific Optical Rotation [alpha]D (c=1in DMF/water 1:1, 20C):
-91.0 to 87.0

Storage and Stability:
Store at -20C with dessicants. Protect from light and humidity.

CAS Number:

Molecular Formula:

Molecular Weight:
Purity: 99%
Form: White, crystalline powder

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ellis, D.D., et al., Bio/Technology, 11(1): 84-89 (1993). 2. Bommineni, V.R., et al., Plant Cell Reports, 13(1): 17-23 (1993). 3. Martin, G.C., et al., J. Amer. Soc. Horticult. Sci., 115(46): 686-691 (1990). 4. Restaino, L., et al., J. Food Protect., 53(6): 508-510 (1990). 5. Watkins, W.D., et al., Appl. Environ. Microbiol., 54: 1874-1875 (1988). 6. Delisle, G.J., and Ley, A., J. Clin. Microbiol. 27(4): 778-779 (1989). 7. Gaudet, I.D., et al., Appl. Environ. Microbiol., 62(11): 4032-4035 (1996). 8. Ceibin, B.W., et al., Appl. Environ. Microbiol., 61(11): 3940-3942 (1995). 9. Sartory, D.P., and Howard, L., Letters in Applied Microbiology, 15: 2373-2376 (1992). 10. Brenner, K.P., et al., Abstracts for the Annual Meeting of the American Society of Microbiology (1993). 11. Frampton, E.W., et al., J. Food Protect. 51(5): 402-404 (1988). 12. Van Pouckle, S.O., and Nelis, H.J., Appl. Environ. Microbiol., 63(2): 771-774 (1997).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.