Technical Data
Media
YPD Broth Traditional Formulation w/Peptone (Powder)
Y2076
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Dextrose20
Peptone20
Yeast Extract10
Total:50g/L
500g makes 10 liters
Standard formulation of YPD media base, formulated with peptone, useful for yeast propagation and plating of mutants and transformants.


Directions per Liter: Dissolve 50g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: White to light tan, homogeneous and free flowing powder

Solubility: Yellow to tan, clear, complete

pH: 7.0 1.0


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application references: 1. Ramage, G. et al., Rev Iberoam Micol (2008) 25: 37-40. 2. Ramage, G. et al., (2004) Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 98:53-59. 3. Monteagudo, C. et al.,(2004) J Clin Pathol. 57:598603. 4. Martins, M. et al., Mycopathologia (2009). 5. Zhang, H. and Seide, W. (2002) Nucleic Acids Research 30:1262-1267. 6. Harris, M.H. et al., (2000) Molecular and Cellular Biology 20:3590-3596. 7. Wasilenko, J.L. et al., (2010) Biotechnology Progress 26; 542-547. 8. Blake-Hodek, K. A. et al., (2010) Mol. Biol. Cell 21; 2013-2023.